Ukr.Biochem.J. 2015; Volume 87, #1, January-February

The influence of iron ions on ATP-hydrolases activity of cell membranes of rat colon smooth muscle and kidney

20.05.2015   Без рубрики   No comments

A. A. Kaplia

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: kaplya@biochem.kiev.ua

To elucidate the specific features of the АТР- hydrolases structural resistance in the membrane under the action of the prooxidants: Fe2+ and hydrogen peroxide, and N-ethylmaleimide (NEM)  the colonic smooth muscle (CSM) Na+,K+-AТРase activity was compared with activities of the corresponding Mg2+-АТР-hydrolase and ATP-ases from kidney medullar layer of rats. The inhibition study of the CSM Na+,K+-AТРase by divalent iron shows the decrease of the activity by 30% at 0.1 µM FeSO4 and in the range of 0.1-10 µM – to 45% of residual activity. When comparing with kidney enzyme (represents exclusively α1-isozyme) the CSM Na+,K+-AТРase sensitivity to Fe2+ is reliably higher at its submicromolar concentration. CSM Mg2+-АТРase is much more resistant to iron ions effect, than kidney one. However for two tissues Mg2+-АТРase activi­ty is always more resistant as compared with corresponding Na+,K+-AТРase activity. Against 1 mM EGTA Na+,K+-AТРase and Mg2+-АТРase activities of GMOK and kidneys are equally insensitive to effect of hydrogen peroxide in concentration up to 1 mM. But in the presence of 20 µM FeSO4 in the concentration range of 1 nМ – 1 mM of Н2О2 the Na+,K+-AТРase is inhibited to greater extent, than Mg2+-АТРase activity. NEM sensitivity of the two АТР-hydrolase systems corresponds to prooxidant sensitivity that indicates the distinct importance of SH-groups for their functioning.

It is concluded that Na+,K+-AТРase can serve as a marker of membrane sensitivity to oxidation, Mg2+-АТРase is resistant to oxidation and can be considered as criterion of the oxidation resistance when comparing  membrane enzyme complexes, especially in GMOK.

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