T. О. Veklich, Iu. Iu. Mazur, S. О. Kosterin

Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
e-mail: veklich@biochem.kiev.ua,
yuliya.vorona@gmail.com

Plasma membrane Ca²⁺-pump is one of key proteins, which takes part in Ca²⁺ exchange in smooth muscle cells. It has a lot of diverse functions from control of basal cytoplasmal Ca²⁺ concentration to regulation of proteins  involved in Ca²⁺-dependent signal pathway. Ca²⁺ pump function is often depen­dent on the isoform or even form of alternative splicing. Allowing for a variety of Ca2+-pump functions and properties, which were reviewed in detail in the first part of our review article cycle (Ukr. Biochem. J., 2015; 87(1)), the precise control of the mentioned pump activity is very important for cell functioning­. The other part of this article is dedicated to different regulation factors of smooth muscle plasma membrane Ca²⁺-pump activity: endogenous and exo­genous, biotic and abiotic factors. Special attention is given to literature data and own results about design and the search of selective plasma membrane Ca²⁺-pump inhibitor which would allow examining its functioning in smooth muscle cells more meticulously.

T. O. Veklich, Iu. Iu. Mazur, S. O. Kosterin

Palladin Institue of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: veklich@biochem.kiev.ua; yuliya.vorona@gmail.com

Tight control of cytoplasm Ca²⁺ concentration is essential in cell functioning. Changing of Са²⁺ concentration is thorough in smooth muscle cells, because it determines relaxation/constraint process. One of key proteins which control Са²⁺ concentration in cytoplasm is Mg²⁺,ATP-dependent plasma membrane calcium pump. Thus, it is important to find compoumds which allowed one to change  Mg²⁺,ATP-dependent plasma membrane calcium pump activity, as long as this topic is of current interest in biochemical research which regards energy and pharmacomechanical coupling mechanism of muscle excitation and contraction. In this article we generalized literatute and own data about properties of smooth muscle cell plasma membrane Ca²⁺— pump. Stuctural oganization, kinetical properties and molecular biology are considered.